The technique

Nuclear magnetic resonance (NMR) is a physical phenomenon based upon the quantum mechanical magnetic properties of an atom's nucleus. NMR also commonly refers to a family of scientific methods that exploit nuclear magnetic resonance to study molecules.

All nuclei that contain odd numbers of protons or neutrons have an intrinsic magnetic moment and angular momentum. The most commonly measured nuclei are hydrogen -1 (the most receptive isotope at natural abundance) and carbon-13, although nuclei from isotopes of many other elements (e.g. 15N 14N 19F, 31P, 17O, 29Si, 10B, 11B, 23Na, 35Cl, 195Pt) can also be observed.

NMR resonant frequencies for a particular substance are directly proportional to the strength of the applied magnetic field, in accordance with the equation for the Larmor precession frequency.

NMR studies magnetic nuclei by aligning them with an applied constant magnetic field and perturbing this alignment using an alternating magnetic field, those fields being orthogonal.The resulting response to the perturbing magnetic field is the phenomenon that is exploited in NMR spectroscopy and magnetic resonance imaging, which use very powerful applied magnetic fields in order to achieve high resolution spectra, details of which are described by the Zeeman effect. 

Applications

Nuclear magnetic spectroscopy is in a wide range of research projects in chemistry, pharmacy, biochemistry, structural biology and physics. Its primary use is in structural characterisation of molecules, from small molecules to proteins over 100,000 Da. 

Facilities Available

Bruker Avance 500, 400 and 300 MHz high resolution spectrometers, These high-field facilities, 400 and 500 MHz, are available to research groups within the school, and from external institutions on a collaborative basis. The low-field facility, (300 MHz)  is used for undergrduate teaching and for research.

Sample requirements and analysis of data


Please contact Dr. Le (details below)

Contacts

SCMB NMR Facility, Room 110, Chemistry Building (#68)
Dr. Tri  Le,  NMR Facility Manager. Contact :3365 4196

email: t.le@uq.edu.au

To access the instruments use the website:
bookingsystem.com. The 400 and 500 MHz  machines can be booked one week in advance, maximium 4 hours per day (between 9am and 7pm) or one overnight per week (start from 7pm to 9am next morning). These instruments can also be booked for a 24 hours period over the week-end. With the 300 machine, during the semester break, the machine will be available for walk-in time from 9am to 5pm each weekday. The user needs to book after 5 pm weekdays or during the week-end. During semester, priority access to the 300 MHz is given to undergraduate teaching. When not needed for teaching, walk-up access is available. For after-hours and the week-end use,  book in advance throught the website.
 

Charges for use

Available on application.

DSCN0401.JPG AVANCE 500
 A Bruker Avance 500 high-resolution NMR spectrometer interfaced to a 11.7 Tesla ULTRASHIELD bore magnet system.

Features - Gradient deuterium shimming
5 mm SELECTIVE EXCITATION INVERSE resonance 1H detect probe with pulsed field gradient facility- 5mm SEI 1H-13C Z-GRD.
High resolution 1H and 13C NMR spectroscopies
2-D spectroscopy: COSY, NOESY, ROESY,TOCSY, HMQC, HMBC, HSQC, DOSY and CLEANEX with and without gradient.
This probe has variable temperature capability
 
DSCN0404.JPG AVANCE 400
A Bruker Avance 400 high-resolution NMR spectrometer interfaced to a 9.4 Tesla bore magnet system.

Features - Gradient deuterium shimming.
5 mm BROAD BAND INVERSE resonance 1H detect probe with pulsed field gradient facility - BBIz PROBE.
High resolution 1H  and 13C spectroscopies.
2-D spectroscopy, COSY, NOESY, ROESY, TOCSY, XHCORR, COLOC, HMQC, HMBC, HSQC, with and without gradient.
Multi-nuclear studies, rare nuclei, e.g. 31P , 13C , 15N, 27Al 
5 mm BROAD BAND OBSERVE resonance 1H detect probe -  BBO PROBE.
High resolution 1H and 13C NMR spectroscopy.
2-D spectroscopy, COSY, NOESY, ROESY,TOCSY, XHCORR, COLOC, HMQC, HMBC.
Multi-nuclear studies, rare nuclei, e.g. 31P , 13C , 15N, 27Al
These two probes have variable temperature capability.
The BBO probe is usually inserted into the spectrometer.
Probe change to the BBIz probe is based on user demand.
DSCN0400_1.JPG AVANCE 300
A Bruker Avance 300 high-resolution NMR spectrometer interfaced to a 7.05 Tesla ULTRASHIELD bore magnet system.

Features - Auto-tuning and matching - Gradient deuterium shimming.
5 mm BROAD BAND OBSERVE resonance 1H detect probe with pulsed field gradient facility- BB0z PROBE.
High resolution 1H and 13C NMR spectroscopies.
2-D spectroscopy, COSY, NOESY, ROESY,TOCSY, XHCORR, COLOC, HMQC, HMBC, HSQC, with and without gradient. 
Multi-nuclear studies, rare nuclei, e.g. 31P , 13C , 15N, 27Al 
This probe has variable temperature capability.
 
DSCN0405.JPG NMR Workstation

 

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