FACS is a specialized type of flow cytometry. Using laser beam to interrogate the flow, cytometer analyses the specific light scattering and fluorescent characteristics of individual cells. Following the cytometry, a stream of cells brakes into droplets and electrically charged. A heterogeneous mixture of biological cells can be electrostatically deflected into containers (tubes or 96 well plate) one cell at a time based upon size and fluorescence.

Charges:
FACS AriaII - $40 for SCMB; $64/hr - non-SCMB memebers

Accuri6 - $15/hr; $24/hr - non-SCMB members

CytoFLEX - $20/hr; $36/hr - non-SCMB members
Consultation is free

Equipment:
BD FACS AriaII equipped with 3 lasers: UV-355nm, Blue-488nm, YG-561nm.

 

 

 

 

 

 

 

 

Accuri6 cytometer equipped with 2 lasers: Blue-488nm, Red-640nm.

 

CytoFLEX flow cytometer equipped with 3 lasers: Violet-405nm, Blue-488nm, Yellow_Green-561nm

 

Detectable Fluorochromes:
HOECHST, DAPI - 355nm; PACBLUE, mCherry - 405nm
SYBR, FITC, PE, EB, PERCP, EGFP - 488nm;
mCherry, PI, PE, CY3, ALEXA, 7-AAD, RHODAMINE - 561nm

more fluorochromes

For spectrum details check here.

FACS Diva and  FLOWJO analysis software and data storage in "cloud " provided.


CytExpert_CytoFlex_flowcytometer_Software

Expertise: We specialize in microbial metagenomic and will assist in subsequent sequence-based analysis of microbial communities (16S profiling and 454 sequencing).

Contact: Dr. Michael Nefedov - m.nefedov@uq.edu.au, telephone 07-336-51833; 0427-428-089

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