Project title: 

Development of targeted nanocarrier delivery system for gene delivery

Project duration:

4-6 weeks

 

Description:

Antisense gene therapy is a technology that uses molecules for treatment of neoplastic diseases. The molecules that are used include antisense oligonucleotides (asODNs) which are short nucleotides. AsODNs act by either activating RNase H or Hybridizing to mRNA and blocking its access to ribosome. Also asODNs can distort DNA transcription. AsODNs are not stable if it is injected directly. Therefore modification works needs to be done. Liposomes are artificial lipid-based vesicles consisted of mono- or bi- lipid layers, which can be used to transport asODNs into the cell.

Chemical coupling of antibodies to the liposomes are needed to make transport more specific. The general objective of this project is to construct and characterize an appropriate nanocarrier (micelle/liposomal based delivery system) in which siRNA can be complexed. In addition to improve  the siRNA delivery to specific organs or cells such as the uterus and thyroid glands through the attachment of peptide, antibody or other ligands to the delivery system in order to increase the uptake of the liposomes, deliver the encapsulated siRNA to specific site in the body and increase its release into the cytoplasm.

Expected outcomes and deliverables:

During this winter project, student will acquire a number of skills related to drug delivery, organic synthesis,  how to use the HPLC, mass spec, NMR, Zeta sizer, also will get good experience in cell culture and how to use Flowcytometry.

Suitable for:

This project is open to applications from students with a background in chemistry (preferably), or biotechnology, or nanotechnology.

Primary Supervisor:

 

Prof Istvan Toth

Further info:

For more details, please contact:

Dr Waleed Hussein

Editor-in-Chief, Vaccination Research Open Journal (VROJ)

Chemistry Building (No. 68, room 1119), Cooper Road

School of Chemistry and Molecular Biosciences

The University of Queensland 

St Lucia, QLD 4072, Australia

Phone (office): +61 7 33469894

Mob: 0411882875; Fax: +61 7 33654273

E-mail: w.hussein@uq.edu.au